Movie 4.

Irreversible GFP-Mem-astrocyte ramifications induced by co-cultured neurons, related to Fig. S2. Neurons precultured for 10 days, followed by co-culture with GFP-Mem-astrocytes for an additional 14 days in the absence or presence of TTX. TTX was added 12 days after the start of co-culture. Merged DIC and fluorescence images were collected as described in Movie 1.