Movie 3.

Live imaging of ΔDsogMS2 Embryos were collected from females carrying MCP-GFP (magenta) and H2Av-RFP (white) mated to males homozygous for the endogenous MS2 live-imaging tag and lacking the distal enhancer. Embryos were imaged from NC12 to NC14 on a Zeiss 880 confocal microscope with each Z-stack taken at 25 second intervals.

Shadow enhancers modulate distinct transcriptional parameters that differentially effect downstream patterning events

Peter H. Whitney, Bikhyat Shrestha, Jiahan Xiong, Tom Zhang, and Christine A. Rushlow

Development 2022. 149:None-None; doi: 10.1242/dev.200940