Movie 10.

Cxcr4a expression in arterial-fated ECs. Maximum intensity projections of confocal z-stacks of Tg(fli1a:nEGFP)^y7; Tg(cxcr4a:YFP)^mu104; Tg(-0.8flt1:RFP)hu⁵³³³ triple transgenic fish labelling all EC nuclei (blue), arterial ECs (red) and cxcr4a positive cells (green) in lateral views anterior to the left. Time-lapse imaging of the formation of posterior circulatory loop. Note the expression of cxr4a in arterial-fated ECs migrating towards the dorsal aorta (white arrowheads). Scale bar: 20 μm.