Movie 11.

Cxcr4a positive ECs display Notch pathway activation. Maximum intensity projections of confocal z-stacks of Tg(fli1a:nEGFP)^y7; Tg(cxcr4a:YFP)^mu104; Tg(TP1:H2B-mcherry)^s939 triple transgenic fish labelling all EC nuclei (blue), cxcr4a positive cells (green) and cells with Notch pathway activation (red) in lateral views anterior to the left. Time-lapse imaging of the formation of posterior circulatory loop. Note activation of Notch signalling and cxcr4a in ECs migrating towards the dorsal aorta (white arrowheads). Scale bar: 30 μm.