Movie 9.

Notch signalling pathway activation in artery-fated ECs. Maximum intensity projections of confocal z-stacks of Tg(fli1a:nEGFP)^y7; Tg(-0.8flt1:RFP)hu⁵³³³; Tg(TP1:Venus-Pest)^s940 triple transgenic fish labelling all EC nuclei (blue), arterial ECs (red) and Notch positive cells (green) in lateral views anterior to the left. Time-lapse imaging of the formation of the posterior circulatory loop. Note activation of Notch signalling in arterial-fated ECs (white arrowheads). Scale bar: 30 μm.