Movie 2.

Primary cilia in the neural tube organoid. The organoids from D6 were immunostained against ARL13B (primary cilia) and acetylated α-tubulin and the confocal images were taken by Zeiss LSM 700 Inverted Microscope with 20X air or 63X oil objective. This result shows the primary cilia expressing ARL13B are localised at the apical membrane of the neural tube organoid which resembles the neural tube in vivo.