Movie 3.

Neural canal-like structure in neural tube organoid. The organoids from D6 were stained against SOX1 (neuroectoderm), Ki67 (proliferating cell), and actin and the images were taken by Zeiss Lightsheet Z1 with 20X water objective. 3D projection of the images (IMARIS, Bitplane) clearly shows the actual tubular structure of the organoid with the hollow and elongated lumen in the middle of the neural tube organoid that recapitulates the neural canal in vivo. Scale bar is 50 μm.