Movie 4.

Interkinetic nuclear migration in the neural tube organoid. Part 1, The organoids from D6 were stained against pH3+ mitotic cells (magenta) and S phase cells (green) and the images were taken by Zeiss Lightsheet Z1 with 20X clearing objective. In both horizontal axis and longitudinal axis, pH3+ mitotic cells were observed at the apical domain and EdU+ S phase cells were observed at the basal domain of the neural tube organoid. This result implies the interkinetic nuclear migration of the cells in the neural tube organoid. Part 2, Time-lapse imaging of the neural tube organoid stained by live cell actin probe with 5 min of interval between D5 and D6 obtained by Visitron CSU-W1 with 60X oil objective. This time-lapse captured the interkinetic nuclear migration of the cells in the neural tube organoid: the cells go to apical domain of the organoid, divide, and reconstitute the epithelium, as the developing neuroepithelial cells do in vivo.

Self-organizing in vitro mouse neural tube organoids mimic embryonic development

JiSoo Park, Hao-An Hsiung, Irina Khven, Gioele La Manno, and Matthias P. Lutolf

Development 2022. 149:None-None; doi: 10.1242/dev.201052