Movie 5.

Maturation of the neural tube organoid. Time-lapse imaging of the neural tube organoids (re-embedded in Matrigel on D6) from D6 to D8 with 1 hour of interval taken by Nikon Eclipse Ti Inverted Microscope with 20X air objective. Part 1, the neural crest cells emerge and migrate out from the neural tube organoid. Part 2, the neurite extends from the neural tube organoid meaning the emergence of mature neurons.