Movie 7.

3D migration of the neural crest cells. Confocal imaging of the neural tube organoids (re-embedded in Matrigel on D6 and cultured until D8) with z-stack shows the neural crest cells migrate out from the organoid three-dimensionally. The images were taken by Zeiss LSM700 inversed with 20X air objective and 40X oil objective. The migrating cells outwards from the organoid expressed neural crest cell markers, namely, NESTIN and SOX10. The 3D view of the Z-stack confocal images was processed on IMARIS (Bitplane).