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Movie 4.

SAC-impaired P. mammillata 256-cell stage embryos undergoing mitosis with and without spindle microtubules. Video microscopy of representative 256-cell stage embryos injected with RNA encoding Mad2-DN and treated with either DMSO (left) or nocodazole (right). Images were acquired every two minutes. Nuclei were visualized by NLS-3xVenus.

Acquisition of the spindle assembly checkpoint and its modulation by cell fate and cell size in a chordate embryo

Marianne Roca, Lydia Besnardeau, Elisabeth Christians, Alex McDougall, Janet Chenevert, and Stefania Castagnetti

Development 2023. 150:None-None; doi: 10.1242/dev.201145