Movie 2.

AQP1-overexpressing cells undergo ectopic cell rounding and detachment. Time-lapse imaging of vitelline vessels in eGFP and AQP1-2A-eYFP-overexpressing embryos at E2. The electroporated region (colored in green), imaged area, and direction of blood flow are illustrated on the left. Freely motile rounded cells are indicated by the arrowheads. The magnified cells in Fig. 4C and Movie 3 are indicated by arrows. Scale bars: 50 μm.

Aquaporin regulates cell rounding through vacuole formation during endothelial-to-hematopoietic transition

Yuki Sato, Mugiho Shigematsu, Maria Shibata-Kanno, Sho Maejima, Chie Tamura, and Hirotaka Sakamoto

Development 2023. 150:None-None; doi: 10.1242/dev.201275