Movie 3.

AQP1-overexpressing cells become rounded along with vacuole formation. Time-lapse imaging of vitelline vessels in eGFP and AQP1-2A-eYFP-overexpressing embryos at E2. The electroporated region (colored in green) and imaged area are illustrated on the left. Ectopic vacuoles (arrows in the right panel) were formed by AQP1 overexpression, leading to ectopic cell rounding and detachment. Scale bars: 5 μm.