Movie 2.
Combined movie of WT (left) and her6, her9 double mutant larvae (right). Combined movie of larvae shown in Fig. 4A (WT; left half) and Fig. 4B (her6-/-, her9-/- double mutant; right half). The movie shows an anti-Sox2 (magenta) and anti-pH3 (green) double immunostaining horizontal image plane confocal stack of 72 hpf WT larvae recorded from a dorsal view. The movie starts with the dorsal-most plane of the image stack and ends with the ventral most plane. Black slides were inserted when one stack had less z-planes than the other stack (here the ventral slides of the WT part). Since mutant and WT have different phenotypic appearances and also differ in size, the planes were aligned according to the thalamic Sox2 expression. This means that more ventral regions might be shifted. Orientation: anterior to the left.