Movie 1.
2D co-culture timelapse imaging. Timelapse optoWnt/WT cocultures under optogenetic stimulation. Three image channels displayed: brightfield (BF, grey), CellTracker membrane dye fluorescence (green), and optoWnt-mVenus-NLS cells (magenta). Time after onset of illumination indicated. Scale bar 250 μm.
Optogenetic control of Wnt signaling models cell-intrinsic embryogenic patterning using 2D human pluripotent stem cell culture
Development 2023. 150:None-None; doi: 10.1242/dev.201386