Movie 2.
2D co-culture timelapse imaging (zoom). Zoom-in of two fields of view of Supplementary Video 2. Left: cell aggregation occurs outside hESC colony; right: cell aggregation occurs inside hESC colony. Merge of CellTracker membrane dye fluorescence (green) and optoWnt-mVenus-NLS cells (magenta). Time after onset of illumination indicated. Scale bar 100 μm.
Optogenetic control of Wnt signaling models cell-intrinsic embryogenic patterning using 2D human pluripotent stem cell culture
Development 2023. 150:None-None; doi: 10.1242/dev.201386