Movie 2.

Maintenance of RFP positive vesicles. Cropped image from a low resolution (10X) long-term light-sheet movie that shows an MCC undergoing basal extrusion and apoptosis , with the RFP positive cluster remaining visible for ∼18HRs until the movie moves out of frame. Movie is 54 frames taken every 20 minutes. Scale is 20μm.

Bidirectional multiciliated cell extrusion is controlled by Notch-driven basal extrusion and Piezo1-driven apical extrusion

Rosa Ventrella, Sun K. Kim, Jennifer Sheridan, Aline Grata, Enzo Bresteau, Osama A. Hassan, Eve E. Suva, Peter Walentek, and Brian J. Mitchell

Development 2023. 150:None-None; doi: 10.1242/dev.201612