Movie 4.

Confocal time-lapse imaging series of a ani-1(RNAi);spd-1(oj5) EMS cell labeled with NMY-2::GFP (in green), and mCherry::PH and mCherry::HIS-58 (in magenta). Temperature was upshifted from 16°C to 22°C, at EMS chromosome condensation (before the video begins). Time is in seconds and time 0 corresponds to anaphase onset. Playback rate is 4 frames per second. Scale bar, 5 μm.