Movie 5.

360° view of 3D reconstruction of volume imaging performed through light sheet fluorescence microscopy at 9.6x of cleared E3 chick embryos electroporated for 24 h (E3 + 24 hpe) with pCS2-mRFP plus pSHIN-ShControl that were whole-mount immuno labeled with anti RFP (magenta) and anti Robo3 (green) antibodies. The RFP channel is shown alone in grey scale after the magenta/green sequence. The image shows 1° steps of a 360° rotation with the axis placed in the cranial-caudal direction. 3D reconstruction was generated with ImageJ/Fiji.