Movie 1.

mRuby3-mCables1 localizes to dI1 growth cones at the floor-plate exit site. Mouse Cables1 (mCables1) was fused to mRuby3 (shown in magenta) and electroporated in ovo into commissural neurons together with a Math1::EGFP-F plasmid to specifically label dI1 neurons (shown in green). This video shows a dI1 growth cone exiting the floor plate and turning rostrally (white arrowhead) in an intact cultured spinal cord using live imaging (see Figue 3A). In the lower right panel a heat map of pseudo-colored mRuby3-mCables together with the edge of the growth cone traced as a white line are shown. A clear signal of mRuby3-mCables could be seen in this growth cone during the exit of the floor plate as well as the rostral turn (white arrowhead). One stack was taken every 10 min for 90 min. Hi, high; Lo, low. Rostral is up.