Movie 1.

Two different hemocyte populations in pupae marked by hml and kn. Representative spinning disc microscopy videos of randomly migrating pupal hml⁺ versus kn⁺ cells expressing an EGFP transgene imaged from a living prepupa (4h APF) as indicated. Right: Co-labeling of migrating kn⁺ cells (green) and hml⁺ plasmatocytes (magenta) in a 16h APF old pupa. Scale bar represents 20 μm. Cells were imaged for 30 minutes. Scale bar represents 20 μm.