Movie 2.
Wholemount Immunostaining of Inner Ear Organoids Derived From DSP-GFP iPSCs. A representative day 80 organoid specimen was wholemount immunostained with antibodies for MYO7A and TUBB3 using a modified SHIELD protocol. The imaged region contains otic organoids with sensory epithelia and dense innervation.
Mapping oto-pharyngeal development in a human inner ear organoid model
Development 2023. 150:None-None; doi: 10.1242/dev.201871