Wholemount Immunostaining of Inner Ear Organoids Derived From DSP-GFP iPSCs. A representative day 80 organoid specimen was wholemount immunostained with antibodies for MYO7A and TUBB3 using a modified SHIELD protocol. The imaged region contains otic organoids with sensory epithelia and dense innervation.
Matthew R. Steinhart, Wouter H. van der Valk, Daniel Osorio, Sara A. Serdy, Jingyuan Zhang, Carl Nist-Lund, Jin Kim, Cynthia Moncada-Reid, Liang Sun, Jiyoon Lee, and Karl R. Koehler
Development
2023. 150:None-None; doi: 10.1242/dev.201871