Movie 6.
Simultaneous imaging of AMPK activity and Ca2+ transients in the axon. Cultured hippocampal neurons were transfected with AMPKAR-EV and jRGECO1a, together with shRNA-scr or shRNA-AMPKα2. The pseudo-color represents the YFP/CFP ratio (AMPK activity, top), and the Ca2+ influx (bottom), respectively. Scale bar: 10 μm. Related to Fig. S4G.
Calcium signals tune AMPK activity and mitochondrial homeostasis in dendrites of developing neurons
Development 2023. 150:None-None; doi: 10.1242/dev.201930