Movie 6.

Simultaneous imaging of AMPK activity and Ca2+ transients in the axon. Cultured hippocampal neurons were transfected with AMPKAR-EV and jRGECO1a, together with shRNA-scr or shRNA-AMPKα2. The pseudo-color represents the YFP/CFP ratio (AMPK activity, top), and the Ca2+ influx (bottom), respectively. Scale bar: 10 μm. Related to Fig. S4G.

Calcium signals tune AMPK activity and mitochondrial homeostasis in dendrites of developing neurons

Akane Hatsuda, Junko Kurisu, Kazuto Fujishima, Ayano Kawaguchi, Nobuhiko Ohno, and Mineko Kengaku

Development 2023. 150:None-None; doi: 10.1242/dev.201930