Movie 2.

Imaging cAMP signalling dynamics in the developmental niche. This move shows similar data to Movie 1, with the contrast optimised to show the dimmer Flamindo2 expressing cells. The undifferentiated, feeding cells are on the left, with the cell aggregation beginning on the right. Towards the right, the population shows synchronous oscillations in Flamindo2 intensity, corresponding to cAMP oscillations (Flamindo2 signal is inversely related to intracellular cAMP level). The playback is at 15 frames per second, with each frame captured every 45 seconds. The field of view is 1.68mm along the long axis.