Movie 1.

JNK Signaling in Spinal Cord Glia. A 12-hour timelapse (1 frame per hour) of an individual GFAP+ glia in a single z plane acquired immediately after spinal cord injury directly anterior to the lesion. The pictured glia can be observed shuttling Venus fluorescence to the cytoplasm multiple times before dividing at the end of the video. The grey channel represents GFAP:cJun-NES-Venus and the red channel represents GFAP:H2A-mCherry. The video frame is oriented with the dorsal side at the top of the frame and the anterior side at the left of the frame.

In toto imaging of glial JNK signaling during larval zebrafish spinal cord regeneration

Clayton J. Becker, Valentina Cigliola, Pierre Gillotay, Ashley Rich, Alessandro De Simone, Yanchao Han, Stefano Di Talia, and Kenneth D. Poss

Development 2023. 150:None-None; doi: 10.1242/dev.202076