Movie 2.

JNK-KTR is Responsive to JNK Inhibition. A 5-hour timelapse (2 frames per hour) of a spinal cord during treatment with 15 μM SP600125. Venus fluorescence can be seen shuttling from the cytoplasm to the nuclear compartment over the course of the video, which is measured as a decrease in JNK activity. The channel on the left represents GFAP:cJun-NES-Venus, and the channel on the right is the corresponding KTR measurement for each frame. Images were taken as a z stack; for visualization purposes they are presented here as a maximum intensity projection, but quantification was performed using individual z levels. The video frame is oriented with the dorsal side at the top of the frame and the anterior side at the left of the frame.

In toto imaging of glial JNK signaling during larval zebrafish spinal cord regeneration

Clayton J. Becker, Valentina Cigliola, Pierre Gillotay, Ashley Rich, Alessandro De Simone, Yanchao Han, Stefano Di Talia, and Kenneth D. Poss

Development 2023. 150:None-None; doi: 10.1242/dev.202076