Movie 3.

Cell Cycling Visualized with pcna^mGFP Zebrafish. A 12 hour timelapse (4 frames per hour) of a spinal cord acquired immediately after spinal cord injury directly anterior to the lesion. GFAP:H2A-mCherry⁺ and GFAP:H2A-mCherrycells can be observed increasing expression of PCNA-mGFP in the center of the frame, which precedes mitosis in a manner described in Fig. 5. The grey channel represents PCNA-mGFP and the red channel represents GFAP:H2A-mCherry. The video frame is oriented with the dorsal side at the top of the frame and the anterior side at the left of the frame.