Movie 4.

Nuclear Movement Visualized with GFAP:H2A-mCherry. A 24 hour timelapse (2 frames per hour) of an uninjured spinal cord starting at 72 hpf and ending at 96 hpf in a single z plane through the center of the spinal cord. Interkinetic nuclear migration can be observed in multiple GFAP:H2A-mCherry⁺ nuclei moving from the dorsal limits of the spinal cord to just dorsal to the central canal (example marked with asterisk). The grey channel represents GFAP:cJun-NES-Venus and the red channel represents GFAP:H2A-mCherry. The video frame is oriented with the dorsal side at the top of the frame and the anterior side at the left of the frame.