Movie 2.

Disrupted secondary sprouting in a tie1 mutant embryo. Time-lapse recording in the trunk of a Tg(kdrl:EGFP);Tg(lyve1:DsRed) tie1^-/- embryo from 37 hpf taken every 30 min by confocal microscopy. Elapsed time (h:min:sec). Lateral view, anterior to the left. In the tie1 mutant, sprouting ECs extend their protrusions but do not migrate dorsally.