Movie 1.

10-plex RNA imaging in a whole-mount zebrafish embryo. Confocal image of 10 RNA targets in the tail of a zebrafish embryo (hbae1, mylpfa, gfap, kdrl, shha, elavl3, sox10, ntla, dmd, col2a1a). Embryo fixed at 27 hpf. See Fig. 2 for additional information.

HCR spectral imaging: 10-plex, quantitative, high-resolution RNA and protein imaging in highly autofluorescent samples

Samuel J. Schulte, Mark E. Fornace, John K. Hall, Grace J. Shin, and Niles A. Pierce

Development 2024. 151:None-None; doi: 10.1242/dev.202307