Movie 1.

Operation overview of Acto3D. Acto3D enables effortless manipulation of opacity, colour tones, and transfer functions in multi-channel fluorescent images, facilitating comprehensive 3D visualisation with optimal processing speed even on a laptop. The shaders governing rendering techniques and transfer functions, including multiplanar reconstruction (MPR), can be instantaneously toggled, enhancing adaptability during visualisation.

Acto3D: an open-source user-friendly volume rendering software for high-resolution 3D fluorescence imaging in biology

Naoki Takeshita, Shinichiro Sakaki, Rie Saba, Satoshi Inoue, Kosuke Nishikawa, Atsuko Ueyama, Yoshiro Nakajima, Kazuhiko Matsuo, Masaki Shigeta, Daisuke Kobayashi, Hideya Yamazaki, Kei Yamada, Tomoko Iehara, and Kenta Yashiro

Development 2024. 151:None-None; doi: 10.1242/dev.202550