Movie 1.
Operation overview of Acto3D. Acto3D enables effortless manipulation of opacity, colour tones, and transfer functions in multi-channel fluorescent images, facilitating comprehensive 3D visualisation with optimal processing speed even on a laptop. The shaders governing rendering techniques and transfer functions, including multiplanar reconstruction (MPR), can be instantaneously toggled, enhancing adaptability during visualisation.
Acto3D: an open-source user-friendly volume rendering software for high-resolution 3D fluorescence imaging in biology
Development 2024. 151:None-None; doi: 10.1242/dev.202550