Movie 2.
HCRTM analysis of a Tbx3Δ/Δc mouse limb bud at E10.5 showing the spatial expression Tbx3 (red), Gli3 (green) and the transgenic mmhs1586-LacZ reporter (blue). Limb buds are oriented with anterior to the top and posterior to the bottom, proximal to the left and distal to the right. The movies show scans through the z-stacks in ventral to dorsal direction. These scans through the entire limb bud mesenchyme show that the wildtype Tbx3 expression does not significantly overlap the spatially identical Gli3 and LacZ expression domains.