Movie 1.
Classifying cell division events in raw movies. Top left; E-Cadherin (magenta) and Myosin-II (green) fluorescence confocal movie channels. Top right; best-fit ellipses drawn on top of segmented and tracked cell shapes. Lengths of principal axes are colour-coded blue (1 μm) to green (5.5 μm) to red (10 μm). Bottom left; dumbbell pinch measure for identifying cytokinesis, increasing from zero (blue) to 0.2 (red). Bottom right; final classification of cytokinesis events. Cells are colour-coded by time to cytokinesis from blue (≤−10 mins) to red (≥0 mins). Images and data from Cad/Myo embryo CS_4.
Mechanical stress combines with planar polarised patterning during metaphase to orient embryonic epithelial cell divisions
Development 2024. 151:None-None; doi: 10.1242/dev.202862