Movie 2.

Multimodal views of representative WT E4.5, E5.5, and E6.5 implantation site 3DMOUSEneST scans. From left to right: E4.5, E5.5, and E6.5 WT decidual nests (SHG is shown in gold and its transparent surface rendering in grey). THG (blue) provides context to outline the conceptus (darker ‘hollow’). Scale bars for all are 200 μm. The video shows the following progression: 1. Unedited scan: SHG (gold) includes part of the myometrium and the decidual nest, and THG (blue) surrounds the conceptus and shows uterine tissue context. 2. Decidual nest (SHG in gold) + tissue context (THG in blue). 3. Decidual nest (SHG in gold). 4. Decidual nest (SHG in gold) + transparent surface rendering of the decidual nest (grey). 5. Transparent surface rendering of the decidual nest (grey). 6. Transparent surface rendering of the decidual nest (grey) + tissue context (THG in blue). THG signal has been cropped around the implantation chamber for clearer visual representation. 7. Transparent surface rendering of decidual nest (grey) + a digital slice of SHG (gold)/THG (blue). Digital slices were used for conceptus and decidual nest measurements in Fig. 7. 8. SHG (gold)/THG (blue) digital slice only. AM, antimesometrial; E, embryonic day; M, mesometrial.