Movie 3.
Representative control and Smad1/5 cKO decidual nests at E5.5. From left to right: Smad1/5 control decidual nest, Smad1/5 cKO decidual nest comparable to control in shape, and a poorly developed Smad1/5 cKO decidual nest. SHG decidual nests are shown in gold and decidual nest transparent surface renderings are in grey. Scale bars for all are 200 μm. The video shows the following progression: 1. Decidual nest (SHG in gold). 2. Decidual nest (SHG in gold) + transparent surface rendering of the decidual nest (grey). 3. Transparent surface rendering of the decidual nest (grey). AM, antimesometrial; E, embryonic day; M, mesometrial.
3DMOUSEneST: a volumetric label-free imaging method evaluating embryo–uterine interaction and decidualization efficacy
Development 2024. 151:None-None; doi: 10.1242/dev.202938