Movie 4.

Representative control and Smad2/3 cKO decidual nests at E6.5. From left to right: Smad2/3 control decidual nest and Smad2/3 cKO decidual nest. SHG decidual nests are shown in gold and decidual nest transparent surface renderings are in grey. Scale bars are 300 μm. The video shows the following progression: 1. Decidual nest (SHG in gold). 2. Decidual nest (SHG in gold) + transparent surface rendering of the decidual nest (grey). 3. Transparent surface rendering of the decidual nest (grey). AM, antimesometrial; E, embryonic day; M, mesometrial.

3DMOUSEneST: a volumetric label-free imaging method evaluating embryo–uterine interaction and decidualization efficacy

Audrey Savolainen, Emmi Kapiainen, Veli-Pekka Ronkainen, Valerio Izzi, Martin M. Matzuk, Diana Monsivais, and Renata Prunskaite-Hyyryläinen

Development 2024. 151:None-None; doi: 10.1242/dev.202938