Movie 2.

3D time-lapse imaging of the in vitro implantation process. The organoids were pretreated with or without hormones. Then, the organoids labeled with a red fluorescent were cocultured with blastocysts. The process of in vitro implantation was monitored using time-lapse imaging from 48 hours to 96 hours after the initiation of the co-culture. 3D images were reconstructed by Imaris. Scale bar, 50 μm.