Movie 3.
Representative whole organoid imaging of the implantation positive sample. After coculture with blastocysts, the organoids were cleared with a tissue-clearing reagent, and whole mount immunostaining for E-cadherin was performed to visualize EEC layer. The nuclei were stained with DAPI. GFP positive cells are blastocyst-derived cells. The images were taken by confocal microscopy and reconstructed in 3D. Scale bar, 100 μm.
Establishment of an in vitro implantation model using a newly developed mouse endometrial organoid
Development 2025. 152:None-None; doi: 10.1242/dev.204461