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Movie 3.

Representative whole organoid imaging of the implantation positive sample. After coculture with blastocysts, the organoids were cleared with a tissue-clearing reagent, and whole mount immunostaining for E-cadherin was performed to visualize EEC layer. The nuclei were stained with DAPI. GFP positive cells are blastocyst-derived cells. The images were taken by confocal microscopy and reconstructed in 3D. Scale bar, 100 μm.

Establishment of an in vitro implantation model using a newly developed mouse endometrial organoid

Taishi Fujimura, Isao Tamura, Azumi Yoshimura, Toshihide Yoneda, Hitomi Takasaki, Amon Shiroshita, Yuichiro Shirafuta, Shun Sato, and Norihiro Sugino

Development 2025. 152:None-None; doi: 10.1242/dev.204461