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Movie 1.

Continuous live imaging of T. ni pupal hindwing from 1 h to 24 h APF (0.7%–17% development). Hoechst33342 dye was used as a live nuclear stain and a single SOP nucleus is pseudocolored in blue. A first division resulted in SOP-II and a transient pIIb that apoptose, following a second division that results in a scale- and socket-building cell.

Lepidopteran scale cells derive from sensory organ precursors through a canonical lineage

Ling S. Loh, Kyle A. DeMarr, Martina Tsimba, Christa Heryanto, Alejandro Berrio, Nipam H. Patel, Arnaud Martin, W. Owen McMillan, Gregory A. Wray, and Joseph J. Hanly

Development 2025. 152:None-None; doi: 10.1242/dev.204501