Movie 10.

The effect of elevated medium osmolarity on actin fiber organization. Time-lapse, spinning-disk confocal movie of a regenerating tissue spheroid expressing Lifeact-GFP in the ectoderm, placed in an isotonic medium containing 70 mM sucrose (Fig. 6E). The movie shows a disordered region that rapidly orders and develops a +1 defect (orange labels), that subsequently unbinds into a pair of +1/2 defects (cyan labels). The tissue spheroid stabilizes in a 4x +1/2 defect configuration and elongates along the fibers' direction but does not regenerate. The images show computationally generated projected views of the fiber organization at the ectodermal basal surface (left) and the cellular organization at the ectodermal apical surface (right). The images were centered to correct for movements of the whole tissue. The elapsed time from excision is displayed (hh:mm), and the scale bar is 100 μm.