Movie 5.

The formation of a rupture hole during a large stretching event at the focus of the actin fiber organization. High-resolution time-lapse, spinning-disk confocal movie acquired every 20 seconds showing the formation of a rupture hole in a regenerating Hydra expressing Lifeact-GFP in the ectoderm (Fig. 4B). The rupture occurs during a large stretching event, initiating as an elongated crack along ectodermal cell-cell junctions, rapidly becoming circular, and sealing back within minutes. The movie shows additional large stretching events (indicated by green frames), with mechanical strain focusing recurring at the same location, at the focus site of the ectodermal actin fibers. The events are slowed down to better demonstrate the tissue deformations. The images show computationally generated projected views of the fiber organization at the ectodermal basal surface (left) and the cellular organization at the ectodermal apical surface (right). The elapsed time from excision is displayed (hh:mm:ss), and the scale bar is 100 μm.