Movie 6.

An extremely large rupture hole in a regenerating tissue spheroid. Time-lapse, spinning-disk confocal movie showing a regenerating tissue spheroid expressing Lifeact-GFP in the ectoderm that experiences two extremely large rupture holes lasting for an extended period of time (∼1 hour; Fig. 4C). The movie shows that the tissue spheroid is nevertheless able to successfully regenerate into a mature Hydra. The rupture holes form at the +1 defect region at the future head site (orange labels). Large deformation events involving mechanical strain focusing and rupture hole formation recur at the same location and are indicated by green frames, and are slowed down to better demonstrate the tissue deformations. Later, large deformation events that are observed from the side (since the tissue rotated and the foci regions are not visible) are indicated by light green frames. The images show the fiber organization at the basal ectodermal surface (left) and the cellular organization at the apical ectodermal surface (right). The images show the computationally generated projected views of fiber organization at the ectodermal basal surface (left) and the cellular organization at the ectodermal apical surface (right). The images were centered to correct for movements of the whole tissue. The elapsed time from excision is displayed (hh:mm), and the scale bar is 100 μm.