Movie 9.

The effect of iCRT14 treatment on actomyosin fiber organization. Time-lapse, spinning-disk confocal movie of a regenerating spheroid expressing Lifeact- GFP in the ectoderm, that was treated with 5 μM iCRT14 (Fig. 6B). The tissue spheroid was initially oriented with its closure region facing the objective. The actomyosin fibers do not reform in the closure region and point defects do not emerge, whereas the inherited aligned fibers visible around the closure region are maintained. The images show computationally generated projected views of the fiber organization at the ectodermal basal surface (left) and the cellular organization at the ectodermal apical surface (right). The images were centered to correct for movements of the whole tissue. The elapsed time from excision is displayed (hh:mm), and the scale bar is 100 μm.