The negative geotaxis test demonstrated locomotor deficits upon downregulation of Fitm preferentially in skeletal muscle
Negative geotaxis assay for Fitm knockdown by the Mef2-GAL4 driver, preferentially expressed in skeletal muscle, in the Fitm RNAi-lines 1A, 1B and 2. The capability of flies to climb on the vial walls is reduced as compared to the respective genetic background controls at 4 and 12 days after eclosion. The video was edited with iMovie (Apple). At least 50 flies were tested per condition and the experiment was replicated twice.