Movie S1
Mouse bone marrow cells follow the migratory route of endogenous primitive macrophages (439 KB). Mouse bone marrow cells from a UBI-GFP transgenic mouse (ubiquitous GFP expression) were isolated, lineage depleted, labeled with CellTrace Violet and transplanted into a ubi:mcherry transgenic fish (ubiquitous mCherry expression). The video was made by compiling images taken every 16 minutes for 13 hours, starting at ~20 hpf. Mouse cells (labeled in green and blue) can be seen migrating over the yolk towards the posterior trunk, a route also followed by endogenous primitive macrophages. The timing of the video is shown in the lower right corner.
Generation of mouse-zebrafish hematopoietic tissue chimeric embryos for hematopoiesis and host-pathogen interaction studies
Disease Models and Mechanisms 2018. 11:None-None; doi: 10.1242/dmm.034876