Live mouse cells circulate within the fish vasculature in xenografted animals (5.5 MB). Mouse bone marrow cells were isolated, lineage depleted, labeled with CellTrace Violet and transplanted into wild type embryos. The video was made by compiling images under epifluorescence illumination at 450 nm taken approx. every 2.3 seconds for 1.8 minutes, starting at ~48 hpf. Live visualization shows mouse cells in circulation throughout the fish body. The timing of the video is shown in the upper right corner.
Margarita Parada-Kusz, Cristina Penaranda, Elliott J. Hagedorn, Anne Clatworthy, Anil V. Nair, Jonathan E. Henninger, Christoph Ernst, Brian Li, Raquel Riquelme, Humberto Jijon, Eduardo J. Villablanca, Leonard I. Zon, Deborah Hung, and Miguel L. Allende
Disease Models and Mechanisms
2018. 11:None-None; doi: 10.1242/dmm.034876