Movie S2

Live mouse cells circulate within the fish vasculature in xenografted animals (5.5 MB). Mouse bone marrow cells were isolated, lineage depleted, labeled with CellTrace Violet and transplanted into wild type embryos. The video was made by compiling images under epifluorescence illumination at 450 nm taken approx. every 2.3 seconds for 1.8 minutes, starting at ~48 hpf. Live visualization shows mouse cells in circulation throughout the fish body. The timing of the video is shown in the upper right corner.

Generation of mouse-zebrafish hematopoietic tissue chimeric embryos for hematopoiesis and host-pathogen interaction studies

Margarita Parada-Kusz, Cristina Penaranda, Elliott J. Hagedorn, Anne Clatworthy, Anil V. Nair, Jonathan E. Henninger, Christoph Ernst, Brian Li, Raquel Riquelme, Humberto Jijon, Eduardo J. Villablanca, Leonard I. Zon, Deborah Hung, and Miguel L. Allende

Disease Models and Mechanisms 2018. 11:None-None; doi: 10.1242/dmm.034876