Movie S5

Long-term dynamics of mouse cells in the fish caudal hematopoietic tissue (3.4 MB). Mouse bone marrow cells were isolated, lineage depleted, labeled with CellTrace Violet and transplanted into nacre-/-, flk1:dsred embryos. The video was made by compiling images taken every 5 minutes for 1 hour and 25 minutes. Pseudo-colored confocal imaging shows mouse cell (green) dynamic displacements and interactions within the fish CHT (red) in a 2 dpf embryo. The lower panel shows only the mouse cells labeled. The timing of the video is shown in the upper right corner.

Generation of mouse-zebrafish hematopoietic tissue chimeric embryos for hematopoiesis and host-pathogen interaction studies

Margarita Parada-Kusz, Cristina Penaranda, Elliott J. Hagedorn, Anne Clatworthy, Anil V. Nair, Jonathan E. Henninger, Christoph Ernst, Brian Li, Raquel Riquelme, Humberto Jijon, Eduardo J. Villablanca, Leonard I. Zon, Deborah Hung, and Miguel L. Allende

Disease Models and Mechanisms 2018. 11:None-None; doi: 10.1242/dmm.034876