Movie S7

Xenotransplanted mouse bone marrow cells respond to bacterial infection in vivo (11.5 MB). Mouse bone marrow cells were isolated, depleted of mature hematopoietic cells, labeled with CellTrace Violet and transplanted into nacre-/-, flk1:dsred embryos. To visualize mouse cell dynamics after a bacterial infection, a 3 dpf xenotransplanted embryo was injected with ~100 cfu of K. pneumoniae (pRSET-tdTomato) into the tail dorsal musculature. The video was made by compiling images taken every 5 minutes for 5 hours and 35 minutes. Live imaging right after the infection shows endogenous fish (red) cells interacting with bacteria at the injection site, while mouse cells display dramatic changes in their distribution within the CHT. The lower panel shows only mouse cells. The timing of the video is shown in the upper right corner. cfu: colony forming units.