Movie S8

Mouse bone marrow derived cells respond to infection in the fish otic vesicle (1.2 MB). Mouse bone marrow cells were isolated, lineage depleted, labeled with CellTrace Violet and transplanted into nacre-/- embryos. A 2 dpf transplanted embryo was infected with ~500 cfu of K. pneumoniae (pRSET-tdTomato) within the otic vesicle and visualized starting at 1 hpi. The video was made by compiling images taken every 5 minutes for 12 hours. Pseudo-colored images show mouse cells (green) migrating to and interacting with bacterial cells (red). The timing of the video is shown in the upper right corner. Scale bar: 10 μm.