Movie S9

Mouse bone marrow derived cells respond to infection in the fish otic vesicle (6.4 MB). Mouse bone marrow cells were isolated, lineage depleted, labeled with CellTrace Violet and transplanted into nacre-/-, flk1:dsred embryos. A 2 dpf transplanted embryo was infected with ~500 cfu of K. pneumoniae (pRSET-tdTomato) within the otic vesicle and visualized starting at 9 hpi. The video was made by compiling images taken every 5 minutes for 8 hours. Pseudo-coloredimages show mouse cells (green) migrating to and interacting with bacterial cells (red). The timing of the video is shown in the upper right corner. Scale bar: 20 μm

Generation of mouse-zebrafish hematopoietic tissue chimeric embryos for hematopoiesis and host-pathogen interaction studies

Margarita Parada-Kusz, Cristina Penaranda, Elliott J. Hagedorn, Anne Clatworthy, Anil V. Nair, Jonathan E. Henninger, Christoph Ernst, Brian Li, Raquel Riquelme, Humberto Jijon, Eduardo J. Villablanca, Leonard I. Zon, Deborah Hung, and Miguel L. Allende

Disease Models and Mechanisms 2018. 11:None-None; doi: 10.1242/dmm.034876